Document Type

Article

Publication Date

10-2003

Abstract

Specific oligonucleotide hybridization conditions were established for single-cell enumeration of uncultivated TM7 and IO25 bacteria by using clones expressing heterologous 16S rRNA. In situ analysis of human subgingival crevice specimens revealed that a greater proportion of samples from sites of chronic periodontitis than from healthy sites contained TM7 subgroup IO25. In addition, IO25 bacterial cells from periodontitis site samples were more abundant and fourfold longer than IO25 cells from healthy site samples.

Comments

Published in Applied and Environmental Microbiology. 2003 October; 69(10): 6294–6298. doi: 10.1128/AEM.69.10.6294-6298.2003. Copyright © 2003, American Society for Microbiology PMCID: PMC201210

At the time of publication Cleber Ouverney was not yet affiliated with San Jose State University

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