Publication Date

2007

Degree Type

Thesis

Degree Name

Master of Science (MS)

Department

General Engineering

Advisor

Guna Selvaduray

Abstract

Techniques to prepare embolic protection filters for micro computed tomography (micro CT) and subsequent histopathology, and which can eventually be used to bring about tissue characterization in terms of Hounsfield Units (HU), were compared. The techniques evaluated in this study included embedding a specimen each in (a) a paraffin cassette, (b) a polymethyl methacrylate (PMMA) block, (c) paraffin followed by sectioning and mounting on glass slides, and d) PMMA followed by sectioning into 800 mum wafers. Micro CT was performed on these specimens. The images were used to reconstruct and detect the HU of the tissue. Embedding the specimen in paraffin was found to be best for preparing specimens suitable for micro CT and subsequent histopathology. Using this technique, CT images with higher resolution were obtained, with adjacent regions having different pixel values. HUs could be clearly differentiated.

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