Publication Date

Fall 2015

Degree Type

Thesis

Degree Name

Master of Science (MS)

Department

Chemistry

Advisor

Joseph Pesek

Subject Areas

Chemistry

Abstract

Silica hydride based stationary phases have demonstrated unique selectivity and specificity for small sized polar molecules. The weakly hydrophobic nature of silica hydride is thought to be the key reason for its remarkable retention capabilities. Traditional stationary phases bonded with fluorine moieties have also exhibited enhanced retention of small polar molecules. In this research, a perfluorinated silica hydride stationary phase was synthesized by hydrosilation and silanization procedures. Chromatographic characterization of the newly packed column was carried out by a large number of retention studies. The separation capabilities of the column were evaluated in both the aqueous normal-phase (ANP) and reversed-phase (RP) modes. The perfluorinated silica hydride based column showed remarkable retention ability for RP and ANP modes of separation. Hydrophilic molecules were strongly retained in the ANP mode and hydrophobic molecules were retained in the RP mode. Effect of varying pH of mobile phase on the retention time was summarized. Use of acetone as non-polar solvent was demonstrated. In the conclusion, silica hydride based perfluorinated stationary phase successfully exhibited dual retention capability for polar and non-polar analytes.

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