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Publication Date

Summer 2013

Degree Type

Thesis - Campus Access Only

Degree Name

Master of Science (MS)


Biological Sciences


Shelley Cargill


analgesics, angiogenesis, estrous, reproduction, transplantation

Subject Areas



Angiogenesis, the formation of new blood vessels, is important for the survival and restoration of functions in ovarian transplantation. Without angiogenesis, transplanted ovarian tissue becomes more susceptible to tissue damage and necrosis. Administration of analgesics for pain management has been shown to decrease angiogenesis, which can influence transplant success. The present study investigated the effects of two analgesics, buprenorphine, an opiate, and meloxicam, a non-steroidal anti-inflammatory drug (NSAID), on the reproductive indicators related to estrous cyclicity and follicular integrity after ovarian transplantation of young ovaries into aged mice. There were no significant differences in estrous cycle length, the onset of estrous cycling, the regularity of estrous cycles, and the proportion of viable follicles and atretic follicles per ovarian sample across treatment groups. Two estrous cycle length analysis methods were compared. Determination of estrous cycle length using the proestrus-to-proestrus method was not ideal as an increased number of individuals had to be excluded from analyses. Determination of estrous cycle length using the proestrus-estrus to proestrus-estrus method was recommended as proestrus can be missed due to its short duration. The former method also resulted in exclusion of more individuals from analyses.