Publication Date

Summer 2018

Degree Type


Degree Name

Master of Science (MS)




Joseph J. Pesek


Column Characterization, HPLC, Resveratrol, Type-C Silica

Subject Areas

Analytical chemistry; Chemistry


High performance liquid chromatography (HPLC) is an analytical technique used to separate, identify and quantify components in a solution based on the affinities between analyte, mobile phase and stationary phase. The main goal of my research is to characterize four Type-C silica columns by comparing the retention of resveratrol analogues. The four columns included are functionalized with either Diamond Bidentate C18 (DBC18), Bidentate C18 (BC18), phenyl hydride or diol groups. Retention maps displaying mobile phase composition compared to compound retention times were compared between columns. In addition to this, two organic solvents (acetonitrile and methanol) were compared. Considering that the resveratrol analogues are fairly nonpolar, this set of compounds were expected to have slightly higher affinity for the DBC18 compared to the BC18. In comparison, the phenyl hydride column was anticipated to interact more with unsaturated compounds while the diol was likely to retain those with polar substituents. The data collected supported some of the expected trends based on polarity but each column demonstrated unique retention mechanisms for specific resveratrol analogues. The results determined that the BC18 column gave the highest retention strength while the phenyl hydride displayed the most selectivity for the resveratrol derivatives.