Master of Science in Bioinformatics (MSBI)
Bisphenol A (BPA), Fragile X Syndrome (FXS), Drosophila melanogaster, Fmr1 gene, mRNA Sequencing, Differential Expression (DE)
Neurological disorders (NDDs), like Fragile X Syndrome (FXS), are becoming more prevalent. One outstanding question in the field is how genetic risk factors for NDDs, like Fmr1, converge with environmental risk factors like bisphenol A (BPA). Our research compares and contrasts the different molecular impacts of BPA exposure in wild-type and FXS flies using mRNA-sequencing analysis. This analysis identifies the mutation in the Fmr1 gene is shown to have a stronger effect on the Drosophila than the presence of BPA, resulting in significant differential gene expression that perturbed several neurological pathways. Moreover, numerous genes responsible for genetic human diseases have homologs in flies. Hence, this analysis has the potential to help better understand and identify novel therapeutic targets.
Raghulan, Rashi, "mRNA-Sequencing Pipeline and Pathway Analysis to Determine the Effects of Bisphenol A on Fragile X Syndrome in Drosophila melanogaster" (2022). Master's Projects. 1201.