Isolation, characterization and antioxidant of polysaccharides from Stropharia rugosoannulata
International Journal of Biological Macromolecules
In this study, polysaccharides from Stropharia rugosoannulata were isolated and purified by macroporous adsorption resin and ion exchange chromatography to produce SRP-1 and SRP-2. Preliminary structural characteristics of SRP-1 and SRP-2 were also investigated. Our results showed that SRP-1 was neutral, while SRP-2 was acidic polysaccharides. The molar ratios of monosaccharides in SRP-1 and SRP-2 were different, but glucose was the main monosaccharide. Both α-type and β-type glycosidic linkages were detected in SRP-1, whereas β-constitution was dominant in SRP-2. Both SRP-1 and SRP-2 had a (1 → 6)-α-D-glucan backbone. Furthermore, the antioxidant activities of polysaccharides against ABTS+, DPPH and OH– radicals were determined in vitro. The results indicated that SRP-2 had a stronger antioxidant capacity than that of SRP-1. Our results may be valuable with regard to the utilization of polysaccharides from S. rugosoannulata as potential pharmaceuticals.
Beijing Advanced Innovation Center for Food Nutrition and Human Health
Antioxidant capacity, Characterization, Isolation, Polysaccharides, Stropharia rugosoannulata
Nutrition, Food Science and Packaging
Ying Liu, Chao Fan Hu, Xi Feng, Lei Cheng, Salam A. Ibrahim, Cheng Tao Wang, and Wen Huang. "Isolation, characterization and antioxidant of polysaccharides from Stropharia rugosoannulata" International Journal of Biological Macromolecules (2020): 883-889. https://doi.org/10.1016/j.ijbiomac.2019.11.045