Application of a Cholesterol-Based Stationary Phase for the Analysis of Brevetoxins

Publication Date

1-1-2023

Document Type

Article

Publication Title

Journal of Separation Science

Volume

46

Issue

2

DOI

10.1002/jssc.202200666

Abstract

A high-performance liquid chromatography protocol for the analysis of brevetoxins has been developed using a silica hydride-based cholesterol column. Brevetoxins are neurotoxins produced by harmful algae that have additional potential as drugs for a number of illnesses/diseases. To develop the optimum conditions, a number of different experimental approaches were tested. These include isocratic and gradient elution, different organic mobile phase components, and temperature variations. A separate protocol was developed for the compounds brevenal and brevenol, also produced by the same algae that make brevetoxins. Brevenal is a natural product under investigation as a therapy for chronic respiratory diseases, such as cystic fibrosis or asthma. The goal of this study was to provide a protocol for the analysis of these compounds that could be further developed into a validated method depending on a particular laboratory's capabilities and to highlight some of the unique features of the cholesterol stationary phase.

Funding Sponsor

University of North Carolina Wilmington

Keywords

potential drug, silica hydride stationary phase, solvent-based selectivity, temperature-based selectivity

Department

Chemistry

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