Application of a Cholesterol-Based Stationary Phase for the Analysis of Brevetoxins
Journal of Separation Science
A high-performance liquid chromatography protocol for the analysis of brevetoxins has been developed using a silica hydride-based cholesterol column. Brevetoxins are neurotoxins produced by harmful algae that have additional potential as drugs for a number of illnesses/diseases. To develop the optimum conditions, a number of different experimental approaches were tested. These include isocratic and gradient elution, different organic mobile phase components, and temperature variations. A separate protocol was developed for the compounds brevenal and brevenol, also produced by the same algae that make brevetoxins. Brevenal is a natural product under investigation as a therapy for chronic respiratory diseases, such as cystic fibrosis or asthma. The goal of this study was to provide a protocol for the analysis of these compounds that could be further developed into a validated method depending on a particular laboratory's capabilities and to highlight some of the unique features of the cholesterol stationary phase.
University of North Carolina Wilmington
potential drug, silica hydride stationary phase, solvent-based selectivity, temperature-based selectivity
Joseph J. Pesek, Maria T. Matyska, Tanya Hiltz, and Jennifer McCall. "Application of a Cholesterol-Based Stationary Phase for the Analysis of Brevetoxins" Journal of Separation Science (2023). https://doi.org/10.1002/jssc.202200666