Growth-Coupled Carotenoids Production Using Adaptive Laboratory Evolution
Publication Date
January 2018
Document Type
Contribution to a Book
Publication Title
Methods in Molecular Biology
Editor
Michael Jensen and Jay Keasling
Volume
1671
DOI
10.1007/978-1-4939-7295-1_20
First Page
319
Last Page
330
Abstract
Adaptive laboratory evolution is a powerful technique for strain development. However, the target phenotypes using this strategy have been limited by the required coupling of the phenotype-of-interest with fitness or survival, and thus adaptive evolution is generally not used to improve product formation. If the desired product confers a benefit to the host, then adaptive evolution can be an effective approach to improve host productivity. In this book chapter, we describe an effective adaptive laboratory evolution strategy for improving product formation of carotenoids, a class of compounds with antioxidant potential, in the yeast Saccharomyces cerevisiae.
Keywords
Adaptive laboratory evolution, Yeast, Carotenoids, Antioxidants, Strain improvement
Recommended Citation
Luis Reyes and Katy Kao. "Growth-Coupled Carotenoids Production Using Adaptive Laboratory Evolution" Methods in Molecular Biology (2018): 319-330. https://doi.org/10.1007/978-1-4939-7295-1_20
