Document Type
Article
Publication Date
10-2003
Volume
69
Issue Number
10
First Page
6294
Last Page
6298
DOI
10.1128/AEM.69.10.6294-6298.2003
Disciplines
Environmental Microbiology and Microbial Ecology | Pathogenic Microbiology
Abstract
Specific oligonucleotide hybridization conditions were established for single-cell enumeration of uncultivated TM7 and IO25 bacteria by using clones expressing heterologous 16S rRNA. In situ analysis of human subgingival crevice specimens revealed that a greater proportion of samples from sites of chronic periodontitis than from healthy sites contained TM7 subgroup IO25. In addition, IO25 bacterial cells from periodontitis site samples were more abundant and fourfold longer than IO25 cells from healthy site samples.
Recommended Citation
Cleber C. Ouverney, Gary C. Armitage, and David A. Relman. "Single-Cell Enumeration of an Uncultivated TM7 Subgroup in the" Faculty Publications, Biological Sciences (2003): 6294-6298. https://doi.org/10.1128/AEM.69.10.6294-6298.2003
Included in
Environmental Microbiology and Microbial Ecology Commons, Pathogenic Microbiology Commons
Comments
Published in Applied and Environmental Microbiology. 2003 October; 69(10): 6294–6298. doi: 10.1128/AEM.69.10.6294-6298.2003. Copyright © 2003, American Society for Microbiology PMCID: PMC201210
At the time of publication Cleber Ouverney was not yet affiliated with San Jose State University