Document Type

Article

Publication Date

January 2002

Publication Title

The Journal of Biological Chemistry

Volume

277

Issue Number

15

First Page

13175

Last Page

13183

DOI

10.1074/jbc.M110809200

ISSN

00219258

Disciplines

Chemical Engineering | Engineering

Abstract

This study characterized the transcript profile of Escherichia coli in acetate cultures using DNA microarray on glass slides. Glucose-grown cultures were used as a reference. At the 95% confidence level, 354 genes were up-regulated in acetate, while 370 genes were down-regulated compared with the glucose-grown culture. Generally, more metabolic genes were up-regulated in acetate than other gene groups, while genes involved in cell replication, transcription, and translation machinery tended to be down-regulated. It appears that E. coli commits more resources to metabolism at the expense of growth when cultured in the poor carbon source. The expression profile confirms many known features in acetate metabolism such as the induction of the glyoxylate pathway, tricarboxylic acid cycle, and gluconeogenic genes. It also provided many previously unknown features, including induction of malic enzymes, ppsA, and the glycolate pathway and repression of glycolytic and glucose phosphotransferase genes in acetate. The carbon flux delivered from the malic enzymes and PpsA in acetate was further confirmed by deletion mutations. In general, the gene expression profiles qualitatively agree with the metabolic flux changes and may serve as a predictor for gene function and metabolic flux distribution.

Comments

SJSU users: Use the following link to login and access the article via SJSU databases.This research was originally published in the Journal of Biological Chemistry. Oh MK, Rohlin L, Kao KC, Liao JC. Global expression profiling of acetate-grown Escherichia coli. J. Biol. Chem. 2002; 277:13175-13183. © the American Society for Biochemistry and Molecular Biology.This article can also be found online here.

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