Publication Date

Fall 2022

Degree Type

Master's Project

Degree Name

Master of Science in Bioinformatics (MSBI)

Department

Computer Science

First Advisor

Wendy Lee

Keywords

Bisphenol A (BPA), Bisphenol F (BPF), Bisphenol S (BPS), endocrine-disrupting compound (EDC), neurodevelopmental disorders (NDDs), RNA-Sequencing (RNA-Seq)

Abstract

Bisphenol A (BPA) is an endocrine-disrupting compound (EDC) that can act as an agonist or antagonist to interfere with multiple signaling pathways, including neurological pathways. The impact of BPA related to neurodevelopmental disorders (NDDs) is one of the biggest concerns in BPA exposure in humans. Therefore, BPA analogues are used to replace BPA in manufacturing and BPA-free products. However, most BPA analogues, including Bisphenol F (BPF) and Bisphenol S (BPS), act the same way as BPA on estrogen and androgen receptors. RNASequencing (RNA-seq) analysis was used to investigate the impact of BPA, BPF, and BPS on neurodevelopmental transcriptomes in Drosophila melanogaster. The results of RNA-Seq analysis identified differentially expressed genes of BPF_exposed and BPS_exposed samples compared to wild-type. The findings of this project found an overall down-regulated gene expression in BPF and BPS_exposed samples compared to wild-type in neurodevelopmental pathways.

Comments

Bisphenol A (BPA) is an endocrine-disrupting compound (EDC) that can act as an agonist or antagonist to interfere with multiple signaling pathways, including neurological pathways. The impact of BPA related to neurodevelopmental disorders (NDDs) is one of the biggest concerns in BPA exposure in humans. Therefore, BPA analogues are used to replace BPA in manufacturing and BPA-free products. However, most BPA analogues, including Bisphenol F (BPF) and Bisphenol S (BPS), act the same way as BPA on estrogen and androgen receptors. RNASequencing (RNA-seq) analysis was used to investigate the impact of BPA, BPF, and BPS on neurodevelopmental transcriptomes in Drosophila melanogaster. The results of RNA-Seq analysis identified differentially expressed genes of BPF_exposed and BPS_exposed samples compared to wild-type. The findings of this project found an overall down-regulated gene expression in BPF and BPS_exposed samples compared to wild-type in neurodevelopmental pathways.

Share

COinS