Publication Date

Spring 2023

Degree Type

Master's Project

Degree Name

Master of Science in Bioinformatics (MSBI)


Computer Science

First Advisor

Wendy Lee

Second Advisor

Cleber Ouverney

Third Advisor

Andreopoulos William


Human microbiome, Metagenomic sequencing, Nanopore sequencing, 16S, Oral microbiome


Human microbiota has a key role in human development and receives considerable attention from research communities. Advances in metagenomics sequencing technologies have significantly improved the capabilities to characterize microbial species associated with human health and disease. Nanopore metagenomics sequencing makes a revolution by detailed characterization of the complex human microbiome. The Nanopore approach resembles traditional genome sequencing but also presents unprecedented challenges. This project will examine the Nanopore approach for oral human microbiome analysis focusing on the 16S rRNA gene collected from the oral cavity of healthy and Mild PKU patients. The findings of this project found that Nanopore sequencing could identify major microbiota at the species level that aligned with many microbiological results. These findings further validate the potential of Nanopore sequencing for analyzing the human microbiome.