Publication Date
Fall 2025
Degree Type
Master's Project
Degree Name
Master of Science in Bioinformatics (MSBI)
Department
Computer Science
First Advisor
Dr. Wendy Lee
Second Advisor
Dr. Theresa Dinh
Third Advisor
Dr. William Andreopoulos
Keywords
ScRNA-seq, PCVs, COUP-TFII, Differential gene expression (DGE), Pathway Analysis
Abstract
High endothelial venules (HEVs) and Post capillary venules (PCVs) are regulators of lymphocyte trafficking in Peyer’s Patches (PP). How chicken ovalbumin upstream promoter transcription factor II (COUP-TFII) distinguishes these closely related venular endothelial types with ETS remains unknown. Using single-cell RNA sequencing of COUP-TFII overexpression and knockout endothelial cells, we identified 10 blood endothelial clusters and observed the altered COUP-TFII and ETS associated pathways across multiple vascular subsets. These findings indicate COUP-TFII modulates venular programs although additional work is required to define the specific regulatory mechanism of PCV identity with respect to ETS.
Recommended Citation
Gupta, Ananya, "Transcriptional Mechanism of Post Capillary Venule (PCVs) Identity in Peyer’s Patches" (2025). Master's Projects. 1620.
https://scholarworks.sjsu.edu/etd_projects/1620
