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Thesis - Campus Access Only
Master of Science (MS)
Joseph J. Pesek
HPLC, Nucleotide separation, UDA column
The UDA column, which has undecynoic acid groups attached to the silica-hydride surface, was chromatographically characterized in this research, and the retention mechanisms are discussed. The polar molecules tested on the UDA column were metabolites (amino acids, carbohydrates), peptides, proteins, nucleosides, nucleotides, and galactosemia biomarkers. Different instrumentation and retention modes were employed depending on the spectroscopic and structural properties of the solutes.
The interactions between the stationary phase and the solutes include both hydrophobic and ionic/hydrophilic interactions. The UDA column possesses a typical aqueous normal phase behavior that can retain both polar and non-polar compounds with a change of mobile-phase composition. The UDA column showed significant retention and separation of most of the hydrophilic compounds, especially nucleotides. Therefore, the UDA stationary phase is classified as an ideal stationary phase for nucleotide separation. However, for other types of compounds, such as a mixture of galactosemia biomarkers, the UDA column was found to be ineffective under the conditions used in this research.
Zhang, Min, "Characterization of a hydride-based HPLC column by polar molecule retention" (2010). Master's Theses. 3839.