Publication Date

Spring 2020

Degree Type


Degree Name

Master of Science (MS)




Joseph J. Pesek


Aqueous Normal Phase, Chromatography, HPLC, Silica Hydride, Stationary Phase, Type C silica

Subject Areas

Analytical chemistry; Chemistry


With many modern chromatographic advancements, silica hydride based columns (also known commercially as Type-C) represent yet another evolutionary step in stationary phase development. Type-C silica hydride columns have the ability to retain both polar and nonpolar analytes and have the ability to behave in either RP or ANP modes of separation depending on mobile phase composition. A consequence of this increased stability is that Type-C columns can be used over a wide range of mobile phase compositions, from purely aqueous to purely nonpolar organic solvents, and any combination of the two. While the retention behavior of Type-C columns depends highly on the mobile phase environment, it can also be influenced by the organic moiety attached to the surface. This project encompasses the evaluation of a silica hydride-based column with amide functionalities to be used in high performance liquid chromatography. A series of separations were performed using reference standards of varying analyte sizes, functionalities, and polarities. This project assesses the specific column selectivity and identifies classes of compounds which show high potential for effective retention, resolution, and efficiency when using amide functionalized silica hydride columns for RP and ANP separations.