Master of Science (MS)
Joseph J. Pesek
Aqueous Normal Phase, Chromatography, HPLC, Silica Hydride, Stationary Phase, Type C silica
Analytical chemistry; Chemistry
With many modern chromatographic advancements, silica hydride based columns
(also known commercially as Type-C) represent yet another evolutionary step in
stationary phase development. Type-C silica hydride columns have the ability to retain
both polar and nonpolar analytes and have the ability to behave in either RP or ANP
modes of separation depending on mobile phase composition. A consequence of this
increased stability is that Type-C columns can be used over a wide range of mobile phase
compositions, from purely aqueous to purely nonpolar organic solvents, and any
combination of the two. While the retention behavior of Type-C columns depends highly
on the mobile phase environment, it can also be influenced by the organic moiety
attached to the surface. This project encompasses the evaluation of a silica hydride-based
column with amide functionalities to be used in high performance liquid chromatography.
A series of separations were performed using reference standards of varying analyte
sizes, functionalities, and polarities. This project assesses the specific column selectivity
and identifies classes of compounds which show high potential for effective retention,
resolution, and efficiency when using amide functionalized silica hydride columns for RP
and ANP separations.
Tardiff, Emma, "Evaluation of Silica Hydride Based Amide Stationary Phase for High Performance Liquid Chromatography and Aqueous Normal Phase Separations" (2020). Master's Theses. 5112.