Image-based analysis and simulation of the effect of platelet storage temperature on clot mechanics under uniaxial strain
Publication Date
2-1-2020
Document Type
Article
Publication Title
Biomechanics and Modeling in Mechanobiology
Volume
19
Issue
1
DOI
10.1007/s10237-019-01203-8
First Page
173
Last Page
187
Abstract
Optimal strength and stability of blood clots are keys to hemostasis and in prevention of hemorrhagic or thrombotic complications. Clots are biocomposite materials composed of fibrin network enmeshing platelets and other blood cells. We have previously shown that the storage temperature of platelets significantly impacts clot structure and stiffness. The objective of this work is to delineate the relationship between morphological characteristics and mechanical response of clot networks. We examined scanning electron microscope images of clots prepared from fresh apheresis platelets, and from apheresis platelets stored for 5 days at room temperature or at 4 °C, suspended in pooled plasma. Principal component analysis of nine different morphometric parameters revealed that a single principal component (PC1) can distinguish the effect of platelet storage on clot ultrastructure. Finite element analysis of clot response to uniaxial strain was used to map the spatially heterogeneous distribution of strain energy density for each clot. At modest deformations (25% strain), a single principal component (PC2) was able to predict these heterogeneities as quantified by variability in strain energy density distribution and in linear elastic stiffness, respectively. We have identified structural parameters that are primary regulators of stress distribution, and the observations provide insights into the importance of spatial heterogeneity on hemostasis and thrombosis.
Funding Number
18AIREA33960524
Funding Sponsor
American Heart Association
Keywords
Fibrin networks, Platelet storage, Principal component analysis, Tensile stress
Department
Chemical and Materials Engineering
Recommended Citation
Sang Joon J. Lee, Dustin M. Nguyen, Harjot S. Grewal, Chaitanya Puligundla, Amit K. Saha, Prajeeda M. Nair, Andrew P. Cap, and Anand K. Ramasubramanian. "Image-based analysis and simulation of the effect of platelet storage temperature on clot mechanics under uniaxial strain" Biomechanics and Modeling in Mechanobiology (2020): 173-187. https://doi.org/10.1007/s10237-019-01203-8
