Direct quantification of single cell drug uptake
Publication Date
1-1-2020
Document Type
Conference Proceeding
Publication Title
24th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2020)
First Page
815
Last Page
816
Abstract
We demonstrate a novel capability to measure drug uptake in single cells using Accelerator Mass Spectrometry (AMS). A microfluidic platform was developed and integrated with our unique moving wire interface, enabling single cells to be measured instantaneously via AMS, in contrast to traditional methods which take multiple days and require large sample sizes. We found evidence of a small subpopulation of cells among a monoculture of breast cancer cells present at 24 hours post chemotherapeutic treatment with a higher amount of drug than other measured cells, suggesting that bulk averages may miss important subpopulations contributing to drug resistance phenotypes.
Funding Number
DE-AC52-07NA27344
Funding Sponsor
U.S. Department of Energy
Keywords
Chemotherapeutic, Droplets, Single Cell
Department
Biomedical Engineering
Recommended Citation
Erika J. Fong, Nick R. Hum, Kelly A. Martin, Melinda Simon, Gaby G. Loots, and Ted J. Ognibene. "Direct quantification of single cell drug uptake" 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2020) (2020): 815-816.
