Direct quantification of single cell drug uptake
24th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2020)
We demonstrate a novel capability to measure drug uptake in single cells using Accelerator Mass Spectrometry (AMS). A microfluidic platform was developed and integrated with our unique moving wire interface, enabling single cells to be measured instantaneously via AMS, in contrast to traditional methods which take multiple days and require large sample sizes. We found evidence of a small subpopulation of cells among a monoculture of breast cancer cells present at 24 hours post chemotherapeutic treatment with a higher amount of drug than other measured cells, suggesting that bulk averages may miss important subpopulations contributing to drug resistance phenotypes.
U.S. Department of Energy
Chemotherapeutic, Droplets, Single Cell
Erika J. Fong, Nick R. Hum, Kelly A. Martin, Melinda Simon, Gaby G. Loots, and Ted J. Ognibene. "Direct quantification of single cell drug uptake" 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2020) (2020): 815-816.