Title

Direct quantification of single cell drug uptake

Publication Date

1-1-2020

Document Type

Conference Proceeding

Department

Biomedical Engineering

Publication Title

24th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2020)

First Page

815

Last Page

816

Abstract

We demonstrate a novel capability to measure drug uptake in single cells using Accelerator Mass Spectrometry (AMS). A microfluidic platform was developed and integrated with our unique moving wire interface, enabling single cells to be measured instantaneously via AMS, in contrast to traditional methods which take multiple days and require large sample sizes. We found evidence of a small subpopulation of cells among a monoculture of breast cancer cells present at 24 hours post chemotherapeutic treatment with a higher amount of drug than other measured cells, suggesting that bulk averages may miss important subpopulations contributing to drug resistance phenotypes.

Funding Number

DE-AC52-07NA27344

Funding Sponsor

U.S. Department of Energy

Keywords

Chemotherapeutic, Droplets, Single Cell

This document is currently not available here.

Share

COinS