Development of a novel microfluidic approach for rapid and continuous detection of pathogens in food and water samples

Publication Date


Document Type

Conference Proceeding

Publication Title

24th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2020)

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We developed a microfluidic device for rapid and high sensitivity detection of pathogens in aqueous samples. We leverage strong preconcentration of isotachophoresis (ITP) and controlled sample flow in microfluidic channels to create a stationary and locally concentrated zone of fluorescently labeled aptamer probes within a microchannel. The labeled bacteria are counted downstream to enable quantitative detection. Our device is the first demonstration of performing stationary ITP in a PDMS microchannel device, rendering high flexibility in the dimensions. We leverage syringe-pump controlled pressure driven flow and parallel channels aiming to achieve monitoring of large sample volumes in the order of mili-liter.


Bacteria, DNA Aptamers, Isotachphoresis, Mirofluidics


Mechanical Engineering; Chemical and Materials Engineering

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