Development of a novel microfluidic approach for rapid and continuous detection of pathogens in food and water samples
Mechanical Engineering; Chemical and Materials Engineering
24th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2020)
We developed a microfluidic device for rapid and high sensitivity detection of pathogens in aqueous samples. We leverage strong preconcentration of isotachophoresis (ITP) and controlled sample flow in microfluidic channels to create a stationary and locally concentrated zone of fluorescently labeled aptamer probes within a microchannel. The labeled bacteria are counted downstream to enable quantitative detection. Our device is the first demonstration of performing stationary ITP in a PDMS microchannel device, rendering high flexibility in the dimensions. We leverage syringe-pump controlled pressure driven flow and parallel channels aiming to achieve monitoring of large sample volumes in the order of mili-liter.
Bacteria, DNA Aptamers, Isotachphoresis, Mirofluidics
Gurpreet Klar, Crystal M. Han, and Liat Rosenfeld. "Development of a novel microfluidic approach for rapid and continuous detection of pathogens in food and water samples" 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2020) (2020): 633-634.