Isotachophoresis-Enhanced Immunoassays: Challenges and opportunities
IEEE Nanotechnology Magazine
Protein detection and quantification is a crucial part of many clinical, research, and preparatory applications . Protein biomarkers, such as prostate-specific antigen (PSA)  and carcinoembryonic antigen (CEA), are regularly detected and quantified for cancer diagnosis and treatment monitoring . On the other hand, cardiac troponin 1 (CTn1) is the most important protein to quantify to detect cardiac injury and myocardial infarction . The detection of protein biomarkers such as PSA, CEA, and CTn1 requires specific identification and quantification of the protein, eliminating detection techniques that enable only size-based quantification of proteins in general, such as silver staining and simple gel electrophoresis .
Ruba Khnouf and Crystal Han. "Isotachophoresis-Enhanced Immunoassays: Challenges and opportunities" IEEE Nanotechnology Magazine (2020): 6-17. https://doi.org/10.1109/MNANO.2020.2966028