ON-CHIP RNA PURIFICATION USING ISOTACHOPHORESIS COUPLED WITH POLYACRYLAMIDE GEL ELECTROPHORESIS
Publication Date
1-1-2021
Document Type
Conference Proceeding
Publication Title
MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences
First Page
1141
Last Page
1142
Abstract
We have developed a method that performs RNA extraction, size selection, and purification in a single microfluidic procedure. We leverage an electric-field based focusing method called isotachophoresis (ITP) and on-chip polyacrylamide gel electrophoresis to overcome the substantial sample loss issues existing in conventional methods. We demonstrate the high resolution and high yield of our method by purifying RNA molecules in the range of 20 - 35 nt with a yield of 80%. Our method is compatible with downstream assays and can be easily customized for different size ranges, which potentially enables sample preparation required for sequencing RNA of specific size from extremely low abundance samples.
Funding Number
CA20452
Funding Sponsor
National Institutes of Health
Keywords
Electrophoresis, Isotachophoresis, Polyacrylamide Gel, RNA Purification
Department
Mechanical Engineering
Recommended Citation
Tori Tonn, Crystal Han, Duc Tran, and Can Cenik. "ON-CHIP RNA PURIFICATION USING ISOTACHOPHORESIS COUPLED WITH POLYACRYLAMIDE GEL ELECTROPHORESIS" MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences (2021): 1141-1142.
