Chromatographic characterization of a silica hydride-based amide stationary phase

Publication Date

7-1-2021

Document Type

Article

Publication Title

Journal of Separation Science

Volume

44

Issue

14

DOI

10.1002/jssc.202100192

First Page

2728

Last Page

2734

Abstract

An amide phase based on a porous silica hydride support material is tested for retention characteristics in both the reversed-phase and aqueous normal-phase modes. A series of retention maps (capacity factor vs. mobile phase composition) were obtained using reference standards of varying analyte sizes, functionalities, and polarities. An assessment of the specific column selectivity is made and classes of compounds are identified that show high potential for effective retention, resolution, and efficiency when using amide functionalized silica hydride columns for reversed-phase and aqueous-normal phase separation. Several practical applications are presented that illustrate the capabilities of this particular column format.

Keywords

aqueous normal phase, drug analysis, retention maps, sugar analysis

Department

Chemistry

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