Structure characterization and in vitro immunomodulatory activities of carboxymethyl pachymaran

Publication Date

5-1-2021

Document Type

Article

Publication Title

International Journal of Biological Macromolecules

Volume

178

DOI

10.1016/j.ijbiomac.2021.02.046

First Page

94

Last Page

103

Abstract

Carboxymethyl pachymaran (CMP) was prepared from Poria cocos polysaccharide by carboxymethylation. Two types of CMP (CMP-1 and CMP-2) were further purified by DEAE-52 anion-exchange chromatography. The structure characteristics and immunomodulatory activities of CMP-1 and CMP-2 were investigated. CMP-1 was determined as β-(1 → 3)-D-glucan. A β-(1 → 3)-D-glucan backbone structure was also found in CMP-2, which was mainly consistent of mannose and glucose, with the mole ratio of 0.03:1. The molecular weight of CMP-1 was 126.1 kDa with a 30.4 nm irregular sphere in distilled water. However, the molecular weight of CMP-2 was 172.6 kDa in a 19.9 nm spherical structure in water solution. Both CMP-1 and CMP-2 had triple helical structure, which can promote the proliferation and the phagocytosis of macrophages. Moreover, CMP-1 and CMP-2 both could improve the secretions of NO, TNF-α and IL-6 by increasing the expression of iNOS, TNF-α and IL-6 mRNA, but CMP-1 exhibited a stronger immunomodulatory ability than that of CMP-2. Our results indicated that CMP-1 and CMP-2 can act as potential immunomodulatory agents.

Funding Number

2016-620-000-001-044

Funding Sponsor

National Key Research and Development Program of China

Keywords

Carboxymethyl pachymaran, Chain conformation, Immunomodulatory, Structure

Department

Nutrition, Food Science and Packaging

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