Publication Date
1-1-2022
Document Type
Article
Publication Title
Forensic Science International: Synergy
Volume
5
DOI
10.1016/j.fsisyn.2022.100291
Abstract
In this study, a quick microwave-based treatment was developed as a front end for DNA analysis of forensic samples. The effect of microwave treatment is to cause cell disruption which can improve the release of DNA during direct PCR as well as with extraction methods. Exposure to microwave preprocessing improved the quality of rapid genotyping, particularly when used with low level samples. Optimal results were obtained when samples were microwaved at 300W for 40 s, resulting in improved allele detection. Overall, the addition of this simple preprocessing step improves sensitivity and allele recovery for low level DNA samples when combined with expedited DNA analysis workflows. Its main advantages include speed, low cost, compatibility with downstream DNA methods and application to a wide variety of samples.
Funding Sponsor
McKnight Foundation
Keywords
Direct PCR, Forensic analysis, Microwave, Microwave extraction, Rapid DNA, Saliva
Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License.
Department
Justice Studies
Recommended Citation
Fabiana Taglia, Ling Wang, Casandra H. Setser, Nicole Fernández-Tejero, Bruce R. McCord, and Steven B. Lee. "Development of a microwave-based extraction for forensic biological samples" Forensic Science International: Synergy (2022). https://doi.org/10.1016/j.fsisyn.2022.100291
