Isoflavone profile and protein molecular weight distribution of soy protein concentrates after soaking treatments

Publication Date

April 2019

Document Type

Article

Publication Title

Journal of Food Processing and Preservation

Volume

43

Issue

4

DOI

10.1111/jfpp.13906

First Page

e13906

Abstract

The effects of soybean soaking at 40, 50, and 60°C, during 4, 8, 12, and 16 hr time periods, on the isoflavone profile and molecular weight distribution of proteins in their soy protein concentrate were evaluated. Results indicated that soaking treatments increased aglycone content (daidzein, genistein, and glycitein) and decreased β‐glucosylated and malonyl glucosylated isoflavone contents. Soy protein concentrate obtained after soaking at 50°C for 16 hr yielded the greatest total aglycone content (5,993.6 µmol 100 g−1), which was 75% greater than the total aglycone content found in soy protein concentrate obtained from non‐treated soybeans (3,636.9 µmol 100 g−1). Glycitein was the main isoflavone aglycone present in soy protein concentrates. Acidic and basic subunits of 11S globulins are released at low and intermediate soaking periods (4, 8, and 12 hr), while longer soaking time (16 hr) causes a protein rearrangement of partially cleaved proteins into more hydrophobic core.

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