Leveraging Single-cell Genomics to Expand the Fungal Tree of Life
Publication Date
October 2018
Document Type
Article
Publication Title
Nature Microbiology
Volume
3
DOI
10.1038/s41564-018-0261-0
First Page
1417
Last Page
1428
Abstract
Environmental DNA surveys reveal that most fungal diversity represents uncultured species. We sequenced the genomes of eight uncultured species across the fungal tree of life using a new single-cell genomics pipeline. We show that, despite a large variation in genome and gene space recovery from each single amplified genome (SAG), ≥90% can be recovered by combining multiple SAGs. SAGs provide robust placement for early-diverging lineages and infer a diploid ancestor of fungi. Early-diverging fungi share metabolic deficiencies and show unique gene expansions correlated with parasitism and unculturability. Single-cell genomics holds great promise in exploring fungal diversity, life cycles and metabolic potential.
Recommended Citation
Steven Ahrendt, C. Alisha Quandt, Doina Ciobanu, Alicia Clum, Asaf Salamov, Bill Andreopoulos, Jan-Fang Cheng, Tanja Woyke, Adrian Pelin, Bernard Henrissat, Nicole Reynolds, Gerald Benny, Matthew Smith, Timothy James, and Igor Grigoriev. "Leveraging Single-cell Genomics to Expand the Fungal Tree of Life" Nature Microbiology (2018): 1417-1428. https://doi.org/10.1038/s41564-018-0261-0
