Publication Date
7-1-2024
Document Type
Article
Publication Title
Foods
Volume
13
Issue
14
DOI
10.3390/foods13142179
Abstract
Lentinula edodes (L. edodes) is a globally popular edible mushroom because of its characteristic sulfur-containing flavor compounds. However, the formation of the volatile sulfur-containing compounds in the mycelium of L. edodes has not been studied. We found that there were also sulfur-containing aroma compounds in the mycelium of L. edodes, and the content and composition varied at different stages of mycelial growth and development. The γ-glutamyl-transpeptidase (GGT) and cysteine sulfoxide lyase (C-S lyase) related to the generation of sulfur compounds showed the highest activities in the 15-day sample. Candidate genes for the metabolism of volatile sulfur compounds in mycelium were screened using transcriptome analysis, including encoding the GGT enzyme, C-S lyase, fatty acid oxidase, HSP20, and P450 genes. The expression patterns of Leggt3 and Leccsl3 genes were consistent with the measured activities of GGT and C-S lyase during the cultivation of mycelium and molecular dynamics simulations showed that they could stably bind to the substrate. Our findings provide insights into the formation of sulfur-containing flavor compounds in L. edodes. The mycelium of L. edodes is suggested for use as material for the production of sulfur-containing flavor compounds.
Funding Number
31601434
Funding Sponsor
National Natural Science Foundation of China
Keywords
KEGG, Lentinula edodes, molecular simulation, mycelium, RNA-seq, sulfur compounds
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 License.
Department
Nutrition, Food Science and Packaging
Recommended Citation
Zheng Li, Fei Pan, Wen Huang, Shuangshuang Gao, Xi Feng, Meijie Chang, Lianfu Chen, Yinbing Bian, Wenli Tian, and Ying Liu. "Transcriptome Reveals the Key Genes Related to the Metabolism of Volatile Sulfur-Containing Compounds in Lentinula edodes Mycelium" Foods (2024). https://doi.org/10.3390/foods13142179
