Establishing an Economical Testing Facility for Genome Sequencing of Chrysaora Plocamia and Human Saliva
Publication Date
1-1-2024
Document Type
Conference Proceeding
Publication Title
2024 IEEE Integrated STEM Education Conference, ISEC 2024
DOI
10.1109/ISEC61299.2024.10665288
Abstract
Genome sequencing is an important method used to identify and analyze organisms' genotypes and biology. Using next generation technologies, Intelliscience Institute, in collaboration with San Jose State University, has set up a fully furnished and economical laboratory capable of sequencing genomes. In order to continue improving the lab's performance, we will analyze new technologies and compare the efficiency and products of major companies in the field. The initial project of sequencing the genome of the Chrysaora plocamia, the South American Sea Nettle Jellyfish, was successfully accomplished using nanopore technology and protocols from New England Biolabs(NEB) and Oxford Nanopore Technology(ONT). The purpose of this work was to sequence a novel marine organism, specifically jellyfish, as they provide key medicinal and ecological information that can aid researchers studying drug development and marine biology. In our current project, we are investigating human saliva. The objective of this research is to investigate how to limit contamination when collecting saliva samples and to analyze the saliva to further study oral microbiomes and hygiene. This work will be done using ZYMO Research protocol for DNA extraction and ONT protocol for library preparation and genome sequencing.
Department
Mechanical Engineering
Recommended Citation
Deshna Shekar, Indeever Madireddy, Prasun Datta, and Sohail Zaidi. "Establishing an Economical Testing Facility for Genome Sequencing of Chrysaora Plocamia and Human Saliva" 2024 IEEE Integrated STEM Education Conference, ISEC 2024 (2024). https://doi.org/10.1109/ISEC61299.2024.10665288
