Cross-species real-time detection of trends in pupil size fluctuation

Publication Date

1-1-2025

Document Type

Article

Publication Title

Behavior Research Methods

Volume

57

Issue

1

DOI

10.3758/s13428-024-02545-7

Abstract

Pupillometry is a popular method because pupil size is easily measured and sensitive to central neural activity linked to behavior, cognition, emotion, and perception. Currently, there is no method for online monitoring phases of pupil size fluctuation. We introduce rtPupilPhase—an open-source software that automatically detects trends in pupil size in real time. This tool enables novel applications of real-time pupillometry for achieving numerous research and translational goals. We validated the performance of rtPupilPhase on human, rodent, and monkey pupil data, and we propose future implementations of real-time pupillometry.

Funding Number

ZIAMH002783

Funding Sponsor

National Institutes of Health

Keywords

Cognitive neuroscience, Cross-species, Pupil, Pupillometry

Department

Audiology

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