Development of an HPLC-Light Scattering Method for the Quantification of Dicyclohexylamine in Beeswax

Publication Date

6-1-2025

Document Type

Article

Publication Title

Separation Science Plus

Volume

8

Issue

6

DOI

10.1002/sscp.70081

Abstract

An excipient present in commercial formulations of fumagillin (an antibiotic used in apiculture for the control of Nosema disease) toxic dicyclohexylamine is found in beeswax samples. There is a need for a reliable analytical method without the use of MS for analysis of this compound. A reversed-phase gradient method was developed for the analysis using a simple extraction procedure followed by low temperature elimination of interfering compounds. Since dicyclohexylamine is lacking a chromophore, an evaporative light scatter detector was used in this analysis. Several challenges of quantitative analysis using an evaporative light scatter detector were addressed. The reported method was validated, robust, sensitive at levels to ensure product safety, accurate, and precise.

Keywords

analyte retention, internal standard, sample preparation, silica hydride stationary phase, universal detector

Department

Chemistry

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