Publication Date
6-1-2025
Document Type
Article
Publication Title
Separation Science Plus
Volume
8
Issue
6
DOI
10.1002/sscp.70081
Abstract
An excipient present in commercial formulations of fumagillin (an antibiotic used in apiculture for the control of Nosema disease) toxic dicyclohexylamine is found in beeswax samples. There is a need for a reliable analytical method without the use of MS for analysis of this compound. A reversed-phase gradient method was developed for the analysis using a simple extraction procedure followed by low temperature elimination of interfering compounds. Since dicyclohexylamine is lacking a chromophore, an evaporative light scatter detector was used in this analysis. Several challenges of quantitative analysis using an evaporative light scatter detector were addressed. The reported method was validated, robust, sensitive at levels to ensure product safety, accurate, and precise.
Keywords
analyte retention, internal standard, sample preparation, silica hydride stationary phase, universal detector
Department
Chemistry
Recommended Citation
Joseph J. Pesek, Maria T. Matyska-Pesek, Tanya Hiltz, and Jason Hall. "Development of an HPLC-Light Scattering Method for the Quantification of Dicyclohexylamine in Beeswax" Separation Science Plus (2025). https://doi.org/10.1002/sscp.70081
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Comments
This is the peer reviewed version of the following article: Pesek, J.J., Matyska-Pesek, M.T., Hiltz, T. and Hall, J. (2025), Development of an HPLC-Light Scattering Method for the Quantification of Dicyclohexylamine in Beeswax. Sep Sci plus., 8: e70081. https://doi.org/10.1002/sscp.70081, which has been published in final form athttps://doi.org/10.1002/sscp.70081. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. This article may not be enhanced, enriched or otherwise transformed into a derivative work, without express permission from Wiley or by statutory rights under applicable legislation. Copyright notices must not be removed, obscured or modified. The article must be linked to Wiley’s version of record on Wiley Online Library and any embedding, framing or otherwise making available the article or pages thereof by third parties from platforms, services and websites other than Wiley Online Library must be prohibited