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Abstract

Forensic entomologists estimate the postmortem interval (PMI) by analyzing insect colonization patterns on remains. Colonization can be influenced by factors such as state of decomposition, environment type, weather, and chemical exposure. Household products and pesticides may be used to conceal remains by delaying decomposition and preventing insect activity. Despite this, their impact on PMI estimation remains unclear. This study examined the effects of 98.11% DEET, 20% picaridin, and 20% IR3535 on insect colonization, compared to a control. Twelve boneless pork chops were placed outdoors in a suburban setting and monitored twice daily at 12-hour intervals. Results showed that high concentrations of DEET (98.11%) and picaridin (20%) delayed colonization for up to 8 days, whereas IR3535 (20%) delayed it slightly. Colonization ranged from low to high levels of larval activity on both the surface and underside of the samples. Further research with larger sample sizes, a longer experimental trial, and varied environmental settings is needed to determine the full impact of pesticides on PMI calculations and whether pesticide exposure should be routinely considered in forensic investigations. Establishing such a relationship could support the development of standardized on-scene pesticide testing procedures and improve PMI accuracy.

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