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Publication Date

Fall 2010

Degree Type

Thesis - Campus Access Only

Degree Name

Master of Science (MS)

Department

Biological Sciences

Advisor

Dr. Cleber C. Ouverney

Subject Areas

Biology; Microbiology; Molecular Biology

Abstract

TM7 is an uncultivable bacterial group. Comparative analysis of the 16S rDNA gene is currently the best way to study such putative division of bacteria. Through this project TM7 16S ribosomal DNA (rDNA) sequences were generated, which were uploaded to open access DNA databases such as the Ribosomal Database Project (RDP) and the National Center for Biotechnology Information (NCBI) database. In addition, during this project a new TM7-specific primer for the polymerase chain reaction (PCR) was designed. PCR based screening helps in the discovery of new uncultivable bacteria like TM7. The TM7 specific primer also helped to distinguish TM7 from other Bacterial domains by allowing greater insight into TM7 phylogeny and the group's evolutionary relationship with other prokaryotes.

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