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Publication Date

Fall 2021

Degree Type

Thesis - Campus Access Only

Degree Name

Master of Science (MS)

Department

Chemical and Materials Engineering

Advisor

Liat Rosenfeld

Subject Areas

Chemical engineering

Abstract

A microfluidic device was developed for rapid and high sensitivity detection of pathogens inwater samples. By leveraging strong preconcentration of isotachophoresis (ITP) and controlled sample flow in microfluidic channels to create a stationary and locally concentrated zone of fluorescently labeled aptamer probes within a microchannel. Two assay solutions were developed for anionic ITP where one contained E. coli DH5⍺ cells and the other contained aptamer AM-6. Counterflow ITP was achieved by incorporating a positive displacement pump into the device. The fabricated device is the first demonstration of performing stationary ITP in a poly(dimethylsiloxane) microchannel device, rendering high flexibility in the dimensions. We leverage syringe-pump controlled pressure driven flow and parallel channels aiming to achieve monitoring of large sample volumes in the order of mili-liter.

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